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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in lots of tumors, which ends up in chromosome instability, and so may perhaps add to tumorigenesis. This protein was uncovered to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a significant role in selling mobile-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, happen to be described. Two supplemental splice variants have been noted but comprehensive nucleotide sequence data is just not however out there.
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Blocking peptides are peptides that bind precisely towards the target antibody and block antibody binding. These peptide normally is made up of the epitope regarded from the antibody.
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This guide outlines even more possible optimization of cellular and immunoassay parameters to be certain the absolute best effects are...
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This guidebook outlines more attainable optimization of cellular and immunoassay parameters to be sure the absolute best benefits are...
Antibodies certain to the blocking peptide no longer bind towards the epitope about the target protein. By comparing the staining within the blocked antibody vs . the antibody by yourself, one can see which staining is specific.
Target Information and facts Cyclin E belongs on the very conserved cyclin household, whose users are characterised by a extraordinary periodicity in protein abundance in the mobile cycle. Cyclins purpose as regulators of CDK kinases. Unique cyclins show unique expression and degradation styles which lead on the temporal coordination of every mitotic occasion. Cyclin E varieties a fancy with and functions for a regulatory subunit of CDK2, whose activity is needed for cell cycle G1/S transition. This protein accumulates on the G1-S phase boundary and is particularly degraded as cells progress via S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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